Short Chain Fatty Acid Panel



Short Chain Fatty Acid Panel

Short-chain fatty acids (SCFAs), especially acetate, propionate and butyrate, are end products of microbial fermentation of dietary fibers from anaerobic intestinal microbiota in the colon. Branched chain fatty acids are mainly derived from protein fermentation and are present in lower amounts.

SCFAs exert multiple beneficial effects on mammalian metabolism. Besides their function as primary energy sources for luminal colon cells, they are involved in different host-signaling mechanisms.

SCFAs play a significant role in:

  • Regulating glucose
  • Lipid metabolism
  • Energy metabolism

And may reduce the risk of developing:

  • Gastrointestinal disorders
  • Cancer
  • Cardiovascular Disease, and more


  • Providing novel insights on how microbiome impacts host’s disease and health status in clinical or experimental studies

  • Evaluating the changes of the host microbiome through dietary, nutraceutical, prebiotics, probiotics, and biopharmaceutical intervention

  • Assessing the homeostasis of gut health wellness

  • Advantages

  • Reproducible Results

  • Fully quantitative LC-MS/MS method with minimum of 6 to 8 calibrators per metabolite

  • Wide range of sample matrices

  • Proprietary technology facilitates low level quantitation in plasma/serum samples

  • Matching stable isotope labeled internal standards for each analyte

  • Analyte panel customizable with additional metabolites such as lactate and other organic acids

  • Calibrator and quality control sample precision (% CV) typically <10%

  • Method

  • Analysis by LC-MS/MS

  • Sciex Exion UHPLC, coupled to a Sciex 5500+ Triple Quadrupole Mass Spectrometer

Panel Analytes

Measured Metabolites
acetate propionate
butyrate isobutyrate
2-methylbutyrate isovalerate
valerate caproate (hexanoate)
lactate* other organic acids*
*available for customization

Sample Requirements

Sample Matrix Sample Amount
Plasma/Serum 200 µL
Feces/Intestinal Content 200 – 500 mg
Bacterial Cultures 200 – 500 µL
Urine 200 – 500 µL
Others upon request

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