Comprehensive Steroid Hormone Panel

Overview
Advantages
Applications
Method

Overview

Steroid Hormone Panel

Steroid hormones play important roles in many physiological processes.

Laboratory tests for numerous steroid hormones, such as testosterone, estradiol, and dehydroepiandrosterone sulfate, are offered from many commercial sources.

However, these tests focus on individual or limited combinations of hormonal species and do not provide a comprehensive picture of the entire steroid hormone biosynthesis.

Applications

  • Deep profiling of steroid hormones could provide valuable insights for research and clinical studies.

Comprehensive panel covering:

  • progestogens

  • mineralocorticoids

  • glucocorticoids

  • androgens

  • estrogens

  • Advantages

  • Reproducible Results

  • Fully quantitative LC-MS/MS method with minimum of 6 to 8 calibrators per analyte

  • Matching stable isotope labeled internal standards for each analyte

  • Calibrator and quality control sample precision (% CV) typically <10%

  • Low sample volume requirement

  • Calibrator and quality control sample precision (% CV) typically <10%

  • Method

  • Analysis by LC-MS/MS

  • Sciex Exion UHPLC, coupled to a Sciex 5500+ Triple Quadrupole Mass Spectrometer

Panel Analytes

Progestogens
progesterone 17α-hydroxyprogesterone
pregnenolone sulfate
Mineralocorticoids
aldosterone deoxycorticosterone
corticosterone 18-hydroxycorticosterone
Glucocorticoids
cortisol cortisone
11-deoxycortisol
Androgens
testosterone androstenedione
11-deoxycortisol dehydroepiandrosterone sulfate (DHEAS)
11-ketotestosterone 11b-hydroxyandrostenedione
Estrogens
estradiol estrone
estrone sulfate

Sample Requirements

Sample Matrix Sample Amount
Plasma/Serum 200 µL

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