Custom Assay Development & Sample Analysis

Experts in Custom biomarker assay development

Precion specializes in the development, validation, and profiling of LC-MS/MS based small molecule biomarker assays.

With decades of experience, the Precion team has developed metabolite biomarkers covering hundreds of metabolites from all chemical classes.

In-house synthesis capabilities for stable isotope labeled internal standards allow for accurate, dependable, and reproducible results.

Precion’s objective in custom assay development is to meet your specific requirements

Precion is flexible in their ability to utilize many different sample types which can easily be scaled in both large and small amounts

Assay validation assures results are accurate and reproducible

Assays are validated following the fit-for-purpose (FFP) approach, with the level of assay characterization determined by the expected content of use of the data
Tested parameters include accuracy, precision, specificity, selectivity, recovery, stability, carry-over, and others as required/requested

Precion leverages LC-MS/MS, the “gold standard” methodology, in quantitative analysis of small molecular biomarkers
Compared to traditional clinical chemistry assays, LC-MS/MS assays are superior in both sensitivity and selectivity
Molecular mass and fragment-mass based detection is universally applicable to the chemically diverse space of small molecules and allows the parallel measurement of many analytes (multiplexing)
Precion specializes in multiplexed assays custom-tailored to address the physiochemical diversity of metabolites
Robust equipment is used for obtaining sensitive, selective, accurate, and precise measurements
Assays are run with a minimum of 6 to 8 calibrators per analyte
Precion uses matching stable isotope labeled internal standards for each analyte, which are synthesized in house if not commercially available
Calibrator and quality control sample precision (% CV) typically <10%
Requires low sample volume