Central Carbon Metabolism & Energy Panel

Overview
Advantages
Applications
Method

Overview

Central Carbon Metabolism & Energy Panel

Central carbon metabolism, also known as the central carbon metabolic network, refers to a series of biochemical pathways that interconnect catabolic and anabolic processes in living cells.

The key central pathways are glycolysis, pentose phosphate cycle and tricarboxylic acid cycle. This metabolic network converts carbon-containing molecules, such as sugars, fatty acids and amino acids into energy as well as precursor molecules necessary for cell growth, proliferation, and survival.

Applications

  • Metabolic clinical research

  • Nutrition research

  • Genetic engineering

  • Bioprocess engineering

  • Advantages

  • Fully quantitative LC-MS/MS method with a minimum of 6 to 8 calibrators per metabolite

  • Wide range of sample matrices

  • Proprietary technology facilitates low level quantitation in plasma/serum samples

  • Matching stable labelled internal standards for each analyte

  • Analyte panel customizable with additional metabolites such as isocitrate and other organic acids

  • Calibrator and quality control sample precision (% CV) typically <10%

  • Method

  • Analysis by LC-MS/MS

  • Sciex Exion UHPLC, coupled to a Sciex 5500+ Triple Quadrupole Mass Spectrometer

Panel Analytes

Measured Metabolites
pyruvate lactate
citrate α-ketoglutarate
succinate malate
fumarate isocitrate*
other organic acids* *available for customization

Sample Requirements

Sample Matrix Sample Amount
Plasma/Serum 100 – 200 µL
Bacterial Cultures 200 – 500 µL
Urine 200 – 500 µL
Others upon request

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