Precion Launches Steroid Hormone Profiling

Steroid hormones play important roles in many physiological processes. Deep profiling of steroid hormones may provide valuable insights for research and clinical studies. Laboratory tests for numerous steroid hormones, such as testosterone, estradiol, and dehydroepiandrosterone sulfate, are offered from many commercial sources. However, these tests focus on individual or limited combinations of hormonal species and do not provide a comprehensive picture of the entire steroid hormone biosynthesis. Also, the amount of sample required to test an extensive panel, in some situations, make it unpractical due to limited sample material.

Key Advantage – Reproducible Results

  • Comprehensive panel covering progestogens, mineralocorticoids, glucocorticoids, androgens, and estrogens

  • Low sample volume requirement, 200 µL

  • Fully quantitative LC-MS/MS method with minimum of 6 to 8 calibrators per analyte

  • Matching stable labelled internal standards used for each analyte

  • Calibrator and quality control sample precision (% CV) typically <10%

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Analytical Method and Instrumentation

  • Analysis by LC-MS/MS

  • Sciex Exion UHPLC, coupled to a Sciex 5500+ Triple Quadrupole Mass Spectrometer

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