Steroid hormones play important roles in many physiological processes. Deep profiling of steroid hormones may provide valuable insights for research and clinical studies. Laboratory tests for numerous steroid hormones, such as testosterone, estradiol, and dehydroepiandrosterone sulfate, are offered from many commercial sources. However, these tests focus on individual or limited combinations of hormonal species and do not provide a comprehensive picture of the entire steroid hormone biosynthesis. Also, the amount of sample required to test an extensive panel, in some situations, make it unpractical due to limited sample material.